
High-recovery one-step purification of the DNA-binding protein Fur by mild guanidinium chloride treatment
Keywords: GdnHCl, guanidinium chloride; PMSF, phenylmethylsulphonyl fluoride; DTT, dithiothreitol; EMSA, electrophoretic mobility shift assays; Px, promoter of the gene X; TCA, tricholoracetic acid; TSP, 3-(trimethylsilyl)propionic acid-d4 sodium SALT; IMAC, Immobi