GPCR-Gα protein precoupling: Interaction between Ste2p, a yeast GPCR, and Gpa1p, its Gα protein, is formed before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain
Keywords: BiFC; bimolecular fluorescence complementation; BRET; bioluminescence resonance energy transfer; C-EGFP; C-terminal domain of enhanced green protein; CFP; cyan fluorescent protein; EGFP; enhanced green fluorescent protein; ER; endoplasmic reticulum; FRET;