The C2 Domains of Classical PKCs are Specific PtdIns(4,5)P2-sensing Domains with Different Affinities for Membrane Binding
Keywords: dDHPE, N-(5-dimethylaminonaphthalene-1-sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine; ECFP, enhanced cyan fluorescent protein; FRET, fluorescence resonance of energy transfer; HBS, Hepes buffer saline; ITC, isothermal titration calorimetry