
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop
Keywords: PTP; protein tyrosine phosphatase; pNPP; p-nitrophenyl phosphate; MUP; 4-methylumbelliferyl phosphate; DiFMUP; 6,8-difluoro-4-methylumbelliferyl phosphate; OPLT; o-phospho-l-tyrosine; HRP; horseradish peroxidase; IPTG; isopropyl-thio-β-d-galactopyranosid