Thermodynamic analysis of Kex2 activity: The acylation and deacylation steps are potassium- and substrate-dependent
Keywords: Abz; ortho-aminobenzoic acid; AMC; [7-amino-4-methyl]coumarin; Bis-Tris; 2,2-Bis(hydroxymethyl)-2,2â²,2â³-nitrilotriethanol; EDDnp; N-[2,4-dinitrophenyl]-ethylenediamine; FRET; fluorescence resonance energy transfer; MES; 2-(N-morpholino)ethanesulfonic