A simple and universal ligation mediated fusion of genes based on hetero-staggered PCR for generating immunodominant chimeric proteins
Keywords: Sea, staphylococcal enterotoxin A; tsst-1, toxic shock syndrome toxin; SOE-PCR, splicing by overlap extension polymerase chain reaction; aa, amino acid(s); LIC, ligation independent cloning; IPTG, isopropyl β-D-thiogalactopyranoside; HRP, horse radish per