
Use of the native flp gene to generate in-frame unmarked mutations in Streptomyces spp.
Keywords: apra; apramycin resistance gene; bp; base pair(s); FLP; Saccharomyces cerevisiae recombinase; FRT; FLP recombinase target; HPLC; high performance liquid chromatography; hyg; hygromycin resistance gene; LB; Luria Broth; MYM; maltose-yeast extract-malt