Recombinant single-chain Fv antibody fragment–alkaline phosphatase conjugate: A novel in vitro tool to estimate rabies viral glycoprotein antigen in vaccine manufacture
Keywords: AP, alkaline phosphatase; BCIP, bromo-chloro-indolyl phosphate; IPTG, isopropyl-β-d-thiogalactopyranoside; MAb, monoclonal antibody; NBT, nitro-blue tetrazolium; PBS, phosphate buffered saline; RV, rabies virus; RVG, rabies virus glycoprotein; scFv, singl