Discovery of a new Fe-type nitrile hydratase efficiently hydrating aliphatic and aromatic nitriles by genome mining
Keywords: NHase, nitrile hydratase; ORFs, open reading frames; OD, optical density; IPTG, isopropyl-β-d-thiogalactopyranoside; DMSO, dimethylsulfoxide; PPB, potassium phosphate bufferNitrile hydratase; Recombinant expression; Substrate specificity; Activator gene;