Molecular expression of l-asparaginase gene from Nocardiopsis alba NIOT-VKMA08 in Escherichia coli: A prospective recombinant enzyme for leukaemia chemotherapy
Keywords: ansA, l-asparaginase biosynthesis gene; IPTG, isopropyl-β-d-thiogalactoside; Ni-NTA, nickel-nitrilotriacetic acid; FT-IR, Fourier transform infrared spectroscopy; NMR, nuclear magnetic resonancel-Asparaginase; Actinobacteria; Nocardiopsis alba; Glutaminas