Hydrolysis of phenylacetanilides catalyzed by penicillin G acylase from Alcaligenes faecalis: Sensitivity of the reaction to substitution in the leaving group
Keywords: Ec-PGA (Af-PGA), penicillin G acylase from Escherichia coli (Alcaligenes faecalis); Ntn, N-terminal nucleophile; TI, tetrahedral intermediate; TS, transition state; AE, acyl-enzyme; GGT, γ-glutamyl transpeptidase; AGA, aspartylglucosaminidasePenicillin G