Engineering of a metagenome derived lipase toward thermal tolerance: Effect of asparagine to lysine mutation on the protein surface
Keywords: CD, Circular dichroism; IPTG, Isopropyl-beta-thio-galactopyranoside; PMSF, Phenylmethylsulphonyl fluoride; DEPC, Diethyl pyrocarbonate; pNP, Para-nitrophenyl; SDS, Sodium dodecyl sulfate; PDB, Protein database; NCBI, National Centre for Biotechnology Info