
The structural analysis and the role of calcium binding site for thermal stability in mannanase
Keywords: Mannanase; Actinomycete; Calcium; Thermal stability; Glucoside hydrolase family 5; CBM; carbohydrate-binding module; DNS; 3,5-dinitrosalicylic acid; ITC; isothermal titration calorimetry; LBG; locust bean gum; PIPES; piperazine-N,Nâ²-bis (2-ethanesulfoni