
Keywords: PMSF، فنیل متیل سولفونیل فلوراید; Heparin; Affinity tag; Single-step; Recombinant proteins and protein purificationHB, heparin-binding; GST, glutathione-S-transferase; GSH, reduced glutathione; IPTG, isopropyl thiogalactopyranoside; PMSF, phenyl methyl sulfonyl fluoride