
The role of substrate specificity and metal binding in defining the activity and structure of an intracellular subtilisin
Keywords: pNA، para nitroanilide; Protease; Subtilisin; Substrate specificity; Metal binding; Binding simulationsESP, extracellular subtilisin; ISP, intracellular subtilisin (processed and active); proISP, unprocessed pro-form of ISP; pNA, para-nitroanilide; proISPS250A, proISP with the a