Determination of the Topology of the Hydrophobic Segment of Mammalian Diacylglycerol Kinase Epsilon in a Cell Membrane and Its Relationship to Predictions from Modeling
Keywords: DGK, diacylglycerol kinase; DGKɛ, epsilon isoform of DGK; TM, transmembrane; PLC, phospholipase C; FLAG, an N-terminal 3×FLAG epitope tag; EDTA, ethylenediaminetetraacetic acid; OG, octylglucoside; PtdIns(4,5)P2, phosphatidylinositol (4,5)-bisphosphate; D