ssDNA hybridization facilitated by T7 ssDNA binding protein (gp2.5) rapidly initiates from the strand terminus or internally followed by a slow zippering step
Keywords: T7 ssDNA binding protein gp2.5; Zipper hybridization; Hybridization of complementary ssDNA; FRET; Rapid quench; The abbreviations used are: 2-AP; 2-aminopurine; Cy3; N,N-(dipropyl)-tetramethylindocarbocyanine; Cy5; N,N-(dipropyl)-tetramethylindodicarbocya