
Thermodynamic principles for the engineering of pH-driven conformational switches and acid insensitive proteins
Keywords: SNase; staphylococcal nuclease; PHS; stable variant of SNase with P117G, H124L, and S128A substitutions; â+PHS; stable variant of SNase consisting of PHS with a 44-49 deletion and additional G50F and V51N substitutions; â+VIAGLA; stable variant of S