Mutation of Gly717Phe in human topoisomerase 1B has an effect on enzymatic function, reactivity to the camptothecin anticancer drug and on the linker domain orientation
Keywords: TBE، تریس بورات EDTA; hTop1B, human topoisomerase 1B; CPT, camptothecin; TPT, topotecan; DMSO, dimethyl sulfoxide; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycol tetraacetic acid; DTT, dithiothreitol; BSA, bovine serine albumin; TBE, tris-borate-EDTA; PMSF, pheny