Introduction of an extra tryptophan fluorophore by cataract-associating mutations destabilizes βB2-crystallin and promotes aggregation
Keywords: آسیب UV; βB2-crystallin; Congenital hereditary cataract; Protein aggregation; Protein folding; Trp fluorophore; UV damage; ANS; 1-anilinonaphtalene-8-sulfonate; CD; circular dichroism; DTT; dithiothreitol; Emax; maximum emission wavelength of fluorescence; IPTG;