A method to accurately quantitate intensities of 32P-DNA bands when multiple bands appear in a single lane of a gel is used to study dNTP insertion opposite a benzo[a]pyrene-dG adduct by Sulfolobus DNA polymerases Dpo4 and Dbh
Keywords: B[a]P; benzo[a]pyrene; +BP-dG; [+ta]-B[a]P-N2-dG (Fig. 1); âBP-dG; [âta]-B[a]P-N2-dG (Fig. 1); DNAP; DNA polymerase; P1; a 20-mer oligonucleotide used in primer-extension assay; P2; a 21-mer oligonucleotide with an additional dC added to the 3â²-end