
Purification and biochemical analysis of catalytically active human cdc25C dual specificity phosphatase
Keywords: cdc25C; Dual-specificity phosphatases; cdk activation; Purification; 3-OMFP; 3-O-methylfluorescein phosphate; BME; β-mercapto-ethanol; C377S; human cdc25C mutated cysteine 377 serine; cdk; cyclin dependent kinase; DTT; dithiothreitol; IPTG; isopropyl β-