کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10026626 1590651 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی انفورماتیک سلامت
پیش نمایش صفحه اول مقاله
B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method
چکیده انگلیسی
To augment the detection of clonality in B-cell malignancies, we designed a consensus primer κ light chain gene (Igκ) polymerase chain reaction (PCR) assay in combination with a consensus primer immunoglobulin heavy chain gene (IgH) PCR assay. Its efficacy was then evaluated in a series of 86 paraffin tissue samples comprising neoplastic and reactive lymphoproliferations. Analysis after PCR was accomplished by 10% native polyacrylamide gel electrophoresis after heteroduplex pretreatment of PCR products and by a post-PCR chip-based capillary electrophoresis analytic method. Overall, 49 of 68 (72%) of mature B-cell neoplasms yielded discrete Igκ gel bands within the predicted size range with no clonotypic Igκ products observed among reactive lymphoid or T-cell proliferations. The application of Igκ PCR improved overall sensitivity from 81% with IgH PCR alone to 90% with combined Igκ/IgH PCR, with this effect being most notable in germinal center-related lymphomas. Sequencing of positive Igκ rearrangements revealed that most rearrangements involved members of the Vκ1 (40%) and Vκ2 (34%) gene families along with Jκ1 (26%), Jκ2 (23%), and Jκ4 (51%) gene segments. Involvement of Vκ pseudogenes was identified in 24% of cases with Vκ-KDE rearrangements. Our results demonstrate the efficacy of Igκ PCR in improving the detection rate of clonality in B-cell neoplasms and further introduce a novel post-PCR chip-based capillary electrophoresis analytic method for rapid PCR fragment size evaluation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Molecular Diagnostics - Volume 7, Issue 2, May 2005, Pages 300-307
نویسندگان
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