Measurement of Whole Blood Tacrolimus Level by High Performance Liquid Chromatography Tandem Mass Spectrometry in Renal Transplant Recipients - A Single Center Perspective

For tacrolimus concentrations within the recommended therapeutic range, the concentration measured by HPLC-MS/MS was statistically significantly lower than that measured by MEIA, but the difference was not clinically significant. Introduction of the more specific HPLC-MS/MS method does not require adjustment in the recommended tacrolimus trough concentration or the AUC12 level estimated by our abbreviated regression equation.