کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10135 667 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing molecular recognition sites on biosurfaces using AFM
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Probing molecular recognition sites on biosurfaces using AFM
چکیده انگلیسی

Knowledge of the molecular forces that drive receptor–ligand interactions is a key to gain a detailed understanding of cell adhesion events and to develop novel applications in biomaterials science. Until recently, there was no tool available for analyzing and mapping these forces on complex biosurfaces like cell surfaces. During the past decade, however, single-molecule atomic force microscopy (AFM) has opened exciting new opportunities for detecting and localizing molecular recognition forces on artificial biosurfaces and on living cells. In this review, we describe the general principles of the AFM technique, present procedures commonly used to prepare samples and tips, and discuss a number of applications that are relevant to the field of biomaterials.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 15, May 2007, Pages 2393–2402
نویسندگان
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