کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10143508 | 1646180 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer
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کلمات کلیدی
qRT-PCRTCGACCK8Small interfering RNA - RNA تداخل کوچکsiRNA - siRNAAkt - آکتThe cancer genome atlas - اوتومتر ژنوم سرطانProliferation - ترویجApoptosis - خزان یاختهایBladder cancer - سرطان مثانهcell counting kit-8 - شمارش سلول کیت 8quantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسprotein kinase B - پروتئین کیناز Bprognosis - پیش شناخت بیماری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer](/preview/png/10143508.png)
چکیده انگلیسی
Bladder cancer (BC) has been regarded as the most common malignancy of the urinary system worldwide. With lack of investigations for molecular pathogenesis underlying that develop BC, the therapeutic efficacy of several therapeutic approaches existing is still unsatisfactory. Here, our study aimed to explore the potentially biological function of MAN1B1 on BC. In this study, MAN1B1 expression level in BC tissues and normal tissues was analyzed based on The Cancer Genome Atlas (TCGA) data and correlation between its expression and prognosis was determined using Kaplan-Meier analysis. Knockout of MAN1B1 was performed using silencing RNA and the efficacy of MAN1B1 knockout was identified using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. The BC cells proliferation was assessed by Cell Counting Kit-8 (CCK8) assay, and then the cells apoptosis was detected by Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) staining and flow cytometry following MAN1B1 knocked down by small interfering RNA. Protein kinase B (AKT) signaling was evaluated by detecting related markers, namely AKT, p-AKT, 4E-BP-1 and Bax using western blot assay. As a result, the MAN1B1 expression was higher in BC tissues than those in normal tissues, besides, its overexpression was associated with poor prognosis. Moreover, MAN1B1 reduction by silencing RNA approach resulted in BC cells proliferation suppression and BC cells apoptosis promotion. Finally, AKT signaling activity was inhibited by MAN1B1 silencing. Taken together, these results unraveled that MAN1B1 may act on an oncogenic action in BC, which improved the likelihood of MAN1B1 taking on a promising prognostic biomarker and a potential target for treating BC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 679, 30 December 2018, Pages 314-319
Journal: Gene - Volume 679, 30 December 2018, Pages 314-319
نویسندگان
Hai-Feng Wang, Jian-Hui Wu, Jun-Wei Gai, Shi-Qiang Yang, Qing-Tong Ma, Hong-Shun Ma, Qiang Feng,