کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10157503 | 1666469 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorylation of androgen receptor serine 81 is associated with its reactivation in castration-resistant prostate cancer
ترجمه فارسی عنوان
فسفولیلاسیون گیرنده آندروژن سریین 81 همراه با فعال شدن آن در سرطان پروستات مقاوم به کاستاریکا است
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کلمات کلیدی
سرطان پروستات، گیرنده آندروژن، فسفریلاسیون، ایمونوهیستوشیمی، رونویسی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Phosphorylation of serine 81 (pS81) in the N-terminal transactivation domain of the androgen receptor (AR) has been linked to its transcriptional activation in prostate cancer (PCa) cell lines, but in vivo studies have been limited. Moreover, the role of pS81 in the reactivation of AR when tumors relapse after androgen deprivation therapy (castration-resistant prostate cancer, CRPC) has not been determined. In this study we validate a pS81 antibody for immunohistochemistry (IHC) and show it yields strong nuclear staining in primary PCa clinical samples and in the VCaP PCa xenograft model. Moreover, this staining was decreased at 7 days post-castration in VCaP xenografts, coinciding with markedly decreased AR transcriptional activity. Staining with the pS81 antibody then was restored when the VCaP xenografts relapsed, which was associated with restoration of AR transcriptional activity. Significantly, analysis of CRPC clinical samples, including tumors that had progressed during treatment with abiraterone, showed strong nuclear staining with the pS81 antibody. Together these findings indicate that AR reactivation in CRPC is associated with S81 phosphorylation, and suggest that IHC for pS81 may be useful as a biomarker of AR activity in CRPC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 438, 1 December 2018, Pages 97-104
Journal: Cancer Letters - Volume 438, 1 December 2018, Pages 97-104
نویسندگان
Joshua W. Russo, Xiaming Liu, Huihui Ye, Carla Calagua, Sen Chen, Olga Voznesensky, James Condulis, Fen Ma, Mary-Ellen Taplin, David J. Einstein, Steven P. Balk, Shaoyong Chen,