کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10157778 | 1666482 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Creatine-loading preserves intestinal barrier function during organ preservation
ترجمه فارسی عنوان
بارگیری کراتین باعث حفظ عملکرد مانع روده در حین حفظ اندام می شود
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کلمات کلیدی
راه حل حفاظت داخلی مواد مغذی خاص روده، بارگیری کراتین، ذخیره سازی سرد
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Our data indicate that creatine loading has significant impact on energy levels during storage with corresponding improvements in mucosal structure and function. Both of our rodent models, a) continuous perfusion for 4â¯h and b) a single flush with our intraluminal preservation solution supplemented with 50â¯mM creatine, demonstrated significant improvements in creatine phosphate, ATP, Energy Charge and ATP/AMP following cold storage (Pâ¯<â¯0.05). Notably, after 10â¯h creatine phosphate was 324% greater in Creatine-treated tissues compared to Controls (Pâ¯<â¯0.05). Preferential utilization of glutathione in the Creatine group was effective at controlling oxidative injury after 10â¯h storage (Pâ¯<â¯0.05). Improvements in barrier function and electrophysiology with creatine-treatment reflected superior mucosal integrity after 10â¯h storage; Permeability and Transepithelial resistance measurements remained at fresh tissue values. This was in stark contrast to Control tissues in which permeability rose to >300% of fresh tissue values (Pâ¯<â¯0.005) and transepithelial resistance dropped by 95% (Pâ¯<â¯0.005). After 10â¯h storage, Park's grading of histologic injury reflected extensive villus denudation (grade 4) in control tissues compared to healthy tissue (grade 0) in the Creatine group. This study demonstrates that a strategy of creatine supplementation of our intraluminal preservation solution facilitates the preservation of the intestinal mucosa during storage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 84, October 2018, Pages 69-76
Journal: Cryobiology - Volume 84, October 2018, Pages 69-76
نویسندگان
Konra Mueller, Matthew S. Kokotilo, Jodi M. Carter, Aducio Thiesen, Karen L. Madsen, Jacek Studzinski, Rachel G. Khadaroo, Thomas A. Churchill,