Zmiany ilościowe limfocytów B i ich subpopulacji CD5+ i CD23+ u dzieci szkolnych z dysplazjÄ oskrzelowo-płucnÄ

IntroductionSome immunological studies in schoolchildren with BPD did not make any concern on B lymphocytes subpopulations.AimTo make an assessment of the quantitative values of B, CD5+ and CD23+ lymphocytes in children with BPD, including the severity of disease.Material and methodsThe study comprised 86 children aged from 8 to 12 years of life, among them 15 with I°, 12 with II°, 9 with III° of BPD and 25 born prematurely and 25 full-term without BPD. The immunological examination comprised determination of leukocytes, lymphocytes, B lymphocytes, CD5+ and CD23+ in peripheral vein blood using FACS scan flow cytometer.ResultsThe mean percentage of B lymphocytes was the lowest in children with BPD III°, significantly lower than in children with BPD I° and II°. It was found significantly higher mean value of CD23+ in children with BPD than in children without BPD. No significant differences were noted in mean values of CD5+ lymphocytes between groups.Conclusions1.In schoolchildren, born preterm with respiratory insufficiency, who became sick with BPD there are quantitative changes of B lymphocytes and their subpopulation CD23+ B.2.The severity of BPD has a crucial influence on the decrease of B lymphocytes and on the increase of CD23+ lymphocytes.3.Quantitative analysis of B lymphocytes and CD23+ lymphocytes in schoolchildren with past severe neonatal pathology may be useful for the assessment of immunological reactions in children with BPD and for determining their immune defences.