Analiza genotypowa oraz profil lekowrażliwości szczepów Staphylococcus aureus izolowanych od dzieci chorych na mukowiscydozę

The mean age of patients studied was 7 years (range 0.5-16 years). Over 80% of patients had pulmonary manifestations. The genotyping confirmed that Staphylococcus aureus persistently colonized the respiratory tract of patients with CF. For over 95% of strains the colonization lasted from 6 months to 8 years. Less than 5% of strains did not show a tendency for a long-term colonization, and they were isolated only once. In 34% of patients the same genotype was isolated through the study period, and it could not be eradicated despite antibiotic treatment. As many as 62% of children were persistently colonized with 2 or 3 strains simultaneously. Over 94% of strains were susceptible to β-lactams, fluoroquinolones, trimethoprim/sulfamethoxazole, aminoglycosides, and glycopeptides. The highest rates of resistance were observed for macrolides (23%) which were the second most commonly used antibiotics in bronchopulmonary exacerbations. Some of S. aureus strains were effectively transmitted among the patients. However, only methicillin-susceptible isolates (MSSA) were involved in the transmission. MRSA transmission was not detected.