کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10227330 | 442 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells
ترجمه فارسی عنوان
پیوند پوستی اپیدرمال بافتی که با سلولهای حاصل از چربی منتقل شده است
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کلمات کلیدی
فیبرین، کلاژن، بافت پوستی مهندسی، آنژیوژنز، بافت چربی، سلول اندوتلیال،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The major problem in skin grafting is that tissue-engineered skin grafts after their transplantation are initially entirely dependent on diffusion. Since this process is slow and inefficient, nutrients, growth factors, and oxygen will insufficiently be supplied and the regenerating graft will undergo a physiological crisis, resulting in scar-like dermal structures and shrinkage. The tissue-engineering of a vascular network in human dermo-epidermal skin substitutes (DESS) is a promising approach to overcome this limitation. Here we report, for the first time, on the use of the adipose stromal vascular fraction (SVF)-derived endothelial cell population to tissue-engineer DESS containing a highly efficient capillary plexus. To develop vascular networks in vitro, we employed optimized 3D fibrin or collagen type I hydrogel systems. Upon transplantation onto immune-deficient rats, these pre-formed vascular networks anastomosed to the recipient's vasculature within only four days. As a consequence, the neo-epidermis efficiently established tissue homeostasis, the dermis underwent almost no contraction, and showed sustained epidermal coverage in vivo. Overall, the here described rapid and efficient perfusion of SVF-based skin grafts opens new perspectives for the treatment of hitherto unmet clinical needs in burn/plastic surgery and dermatology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 19, June 2014, Pages 5065-5078
Journal: Biomaterials - Volume 35, Issue 19, June 2014, Pages 5065-5078
نویسندگان
Agnieszka S. Klar, Sinan Güven, Thomas Biedermann, Joachim Luginbühl, Sophie Böttcher-Haberzeth, Claudia Meuli-Simmen, Martin Meuli, Ivan Martin, Arnaud Scherberich, Ernst Reichmann,