کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228320 | 479 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of electrically charged polyion complex nanoparticle-coated surfaces on adipose-derived stromal progenitor cell behaviour
ترجمه فارسی عنوان
اثر سطوح پوشش نانوذرات پیچیده پلی یون الکتریکی بر رفتار سلول پیش ساز استروما حاصل از چربی
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کلمات کلیدی
نانوذرات، خود سازمان، سلول ها، پلی یونها، مویرگی تجمیع،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Surface characteristics of biomaterials such as wettability, rigidity, roughness, and electrical charge affect the fate of transplanted cells such as progenitor cells or stem cells for use in regenerative medicine. Of these, the effects of surface electrical charges on cellular behaviour such as adhesion, proliferation, and differentiation are not well understood. We prepared precisely charged culture surfaces ranging from â28 mV to +21 mV, simply by surface deposition of polyion complex nanoparticles prepared by mixing a positively charged thermoresponsive homopolymer, poly(N,N-dimethylaminoethyl methacrylate), with negatively charged plasmid DNA at various charge ratios. Drastic morphological changes of adipose-derived vascular progenitor cells were generated on the positively charged surface of organized forms at +19 mV. Capillary-like networks or single aggregates of these cells were selectively created depending on cell seeding density. Our findings offer new insights that may aid develop stem cell-processing techniques for use in regenerative medicine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 36, December 2013, Pages 9096-9102
Journal: Biomaterials - Volume 34, Issue 36, December 2013, Pages 9096-9102
نویسندگان
Ryosuke Iwai, Yasushi Nemoto, Yasuhide Nakayama,