کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228337 | 479 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ probing of intracellular pH by fluorescence from inorganic nanoparticles
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Intracellular pH (pHi) plays a critical role in the physiological processes of cells. Nanoscale sensors based on pH-sensitive fluorescent proteins attached on nanoparticles (NPs) have been designed but inorganic NP-dependent fluorescent nanosensors have not yet been explored. Herein we describe a pH sensitive inorganic semiconductor fluorescent probe based on ultrathin 3C-SiC NPs which can effectively monitor pH in the range of 5.6-7.4 by taking advantage of the linear dependence between the fluorescent intensity ratio of the surface OHâ and H+ bonding states to band-to-band recombination and pH. Detection of pHi is demonstrated in living HeLa cells. In particular, pHi measurements during apoptosis confirm the validity and sensitivity of this technique in monitoring real-time changes in the intracellular environment. Toxicity assessment and confocal laser scanning microscopy indicate that the 3C-SiC NPs have low cytotoxicity and are compatible with living cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 36, December 2013, Pages 9183-9189
Journal: Biomaterials - Volume 34, Issue 36, December 2013, Pages 9183-9189
نویسندگان
Junhong Guo, Shijie Xiong, Xinglong Wu, Jiancang Shen, Paul K. Chu,