کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10228487 483 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of 3D structure and protein conformation on the innate and adaptive immune responses to silk-based biomaterials
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The role of 3D structure and protein conformation on the innate and adaptive immune responses to silk-based biomaterials
چکیده انگلیسی
We have investigated monocyte and T cell responsiveness to silk based biomaterials of different physico-chemical characteristics. Here we report that untransformed CD14+ human monocytes respond to overnight exposure to silk fibroin-based biomaterials in tridimensional form by IL-1β and IL-6, but not IL-10 gene expression and protein production. In contrast, fibroin based materials in bidimensional form are unable to stimulate monocyte responsiveness. The elicitation of these effects critically requires contact between biomaterials and responding cells, is not sustained and becomes undetectable in longer term cultures. We also observed that NF-κβ and p38 MAP kinase play key roles in monocyte activation by silk-based biomaterials. On the other hand, fibroin based materials, irrespective of their physico-chemical characteristics appeared to be unable to induce the activation of peripheral blood T cells from healthy donors, as evaluated by the expression of activation markers and IFN-γ gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 33, November 2013, Pages 8161-8171
نویسندگان
, , , , , , , , , ,