کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228675 | 493 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells
ترجمه فارسی عنوان
اثرات همکاری نانوپوتوگرافی و همکاری با سلولهای اندوتلیال بر استخوان سازی سلول های بنیادی مزانشیمی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Inspired by the aligned nanostructures and co-existence of vascular cells and stem cells in human cancellous bone, we quantitatively investigated the relative contributions of nanotopography and co-culture with human umbilical endothelial cells (HUVECs) to the osteogenesis of human mesenchymal stem cells (hMSCs). Although both nanotopography and co-culture independently enhanced the osteogenesis of hMSCs, osteogenesis was further enhanced by the two factors in combination, indicating the importance of synergistic cues in stem cell engineering. Interestingly, nanotopography provided a larger relative contribution to the osteogenesis of hMSCs than did co-culture with HUVECs. Furthermore, the osteogenesis of hMSCs was also affected by the density of parallel nanogrooves, exhibiting a maximum at a 1:3 spacing ratio, as defined as the ratio of ridge width to groove width. Analysis of (i) biochemical soluble factors, (ii) hMSC-substrate interaction and (iii) hMSC-HUVEC interaction suggests that (ii) and (iii) play a crucial role in mediating osteogenic phenotypes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 30, October 2013, Pages 7257-7268
Journal: Biomaterials - Volume 34, Issue 30, October 2013, Pages 7257-7268
نویسندگان
Jangho Kim, Hong Nam Kim, Ki-Taek Lim, Yeonju Kim, Shambhavi Pandey, Pankaj Garg, Yun-Hoon Choung, Pill-Hoon Choung, Kahp-Yang Suh, Jong Hoon Chung,