کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10228878 497 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein-resistant, reductively dissociable polyplexes for in vivo systemic delivery and tumor-targeting of siRNA
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Protein-resistant, reductively dissociable polyplexes for in vivo systemic delivery and tumor-targeting of siRNA
چکیده انگلیسی
Small interfering RNA (siRNA) has been considered as a very attractive therapeutic alternative to chemical drugs; however, the chemical and biological instability and poor delivery efficiency of siRNA limit its success in clinical applications. Here we report a protein-resistant, reductively dissociable siRNA delivery system based on self-assembled polyelectrolyte complexes of dextran-siRNA conjugates linked by disulfide bonds. The prepared polyplexes exhibit excellent dispersion stability in the presence of serum because of the anti-fouling property of dextran exposed onto the complex surface. The enzymatic degradation of siRNA is also effectively suppressed within the complex. Folates are introduced as an active tumor-targeting moiety via the conjugation of folates to the hydroxyl groups of dextran. An in vivo investigation with a xenograft tumor mouse model shows that the folate-decorated dextran-siRNA conjugates are very efficiently targeted to cancer cells and induce sequence-specific gene silencing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 9, March 2013, Pages 2370-2379
نویسندگان
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