کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10229341 518 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The transfection activity of R8-modified nanoparticles and siRNA condensation using pH sensitive stearylated-octahistidine
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The transfection activity of R8-modified nanoparticles and siRNA condensation using pH sensitive stearylated-octahistidine
چکیده انگلیسی
Emerging evidence indicates that the efficiency of siRNA loading into an RNA-induced silencing complex (RISC) is a major factor in gene silencing at low doses. In particular, the release of siRNA from components delivered to the cytoplasm could be a first step for achieving maximum gene knockdown effect in siRNA delivery vector. To test this hypothesis, we used a stearylated-octahistidine (STR-H8) as a pH responsive polycation that facilitates the efficient release of siRNA into the cytoplasm, while a stearylated-octaarginine (STR-R8) was used as a conventional cationic polycation. As a fundamental structure, we used octaarginine (R8) and GALA, as a pH-sensitive fusogenic peptide, modified lipid envelope-type nanoparticles (R8/GALA-MENDSUV), as reported previously. When STR-H8/siRNA condensed complexes were loaded in the R8/GALA-MENDSUV, the luciferase knockdown effect was significantly increased compared to STR-R8/siRNA condensed complexes in time and dose dependent manners. Quantification of the released siRNA from the condensed complexes demonstrated that only the STR-H8/siRNA released significant levels of siRNA at pH = 7.4, the pH of cytoplasmic, compared with STR-R8/siRNA condensed complexes. In addition, imaging studies indicated that STR-H8/siRNA facilitated siRNA release. Collectively, these data reveal the importance of the controlled release of siRNA to the cytoplasm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 4, January 2013, Pages 1337-1343
نویسندگان
, , ,