کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10229594 545 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanosheet-based titania microspheres with hollow core-shell structure encapsulating horseradish peroxidase for a mediator-free biosensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Nanosheet-based titania microspheres with hollow core-shell structure encapsulating horseradish peroxidase for a mediator-free biosensor
چکیده انگلیسی
Nanosheet-based titania (TiO2) microspheres with a hollow core-shell structure have been synthesized and employed to immobilize horseradish peroxidase (HRP) in order to fabricate a mediator-free biosensor. The morphology and structure of the TiO2 microspheres were characterized by X-ray diffraction, scanning electron microscopy and transmission electronic microscopy. A possible growth mechanism has been proposed. Spectroscopic and electrochemical measurements revealed that the TiO2 microspheres are an immobilization support with biocompatibility for enzymes, affording good enzyme stability and bioactivity. Due to the nanosheet-based hollow core-shell structure of the TiO2 microspheres, the direct electron transfer of HRP is facilitated and the resulting biosensor displayed good performance for the detection of H2O2, with both a low detection limit of 0.05 μM and a wide linear range of 0.4-140 μM, as well as a fast response and excellent long-term stability. The nanosheet-based TiO2 microspheres with hollow core-shell structure, can be used for the efficient entrapment of other redox-active proteins and have wide potential applications in biosensors, biocatalysis, biomedical devices and bioelectronics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 32, Issue 27, September 2011, Pages 6588-6594
نویسندگان
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