کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10229985 | 594 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gadonanotubes as magnetic nanolabels for stem cell detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Stem cell-based therapies have emerged as a promising approach in regenerative medicine. In the development of such therapies, the demand for imaging technologies that permit the noninvasive monitoring of transplanted stem cells in vivo is growing. Here, we report the performance of gadolinium-containing carbon nanocapsules, or gadonanotubes (GNTs), as a new T1-weighted magnetic resonance imaging (MRI) intracellular labeling agent for pig bone marrow-derived mesenchymal stem cells (MSCs). Without the use of a transfection agent, micromolar concentrations of GNTs can deliver up to 109 Gd3+ ions per cell without compromising cell viability, differentiation potential, proliferation pattern, and phenotype. Imaging 10Â ÃÂ 106 GNT-labeled MSCs demonstrates a nearly two-fold reduction in T1 relaxation time when compared to unlabeled MSCs at 1.5Â T in a clinical MRI scanner, which easily permits the discrimination of GNT-labeled MSCs in a T1-weighted MR image. It is anticipated that GNTs will allow in vivo tracking of GNT-labeled MSCs, as well as other mammalian cell types, by T1-weighted imaging with greater efficacy than other current technologies now allow.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 36, December 2010, Pages 9482-9491
Journal: Biomaterials - Volume 31, Issue 36, December 2010, Pages 9482-9491
نویسندگان
Lesa A. Tran, Ramkumar Krishnamurthy, Raja Muthupillai, Maria da Graça Cabreira-Hansen, James T. Willerson, Emerson C. Perin, Lon J. Wilson,