کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10230495 825 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein array method for assessing in vitro biomaterial-induced cytokine expression
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Protein array method for assessing in vitro biomaterial-induced cytokine expression
چکیده انگلیسی
This study demonstrates the feasibility of a cytokine-based in vitro test for biomaterials. The combination of monocyte culture and protein array technology tested in this study permitted the detection of subtle changes in cytokine expression following an exposure to titanium (Ti) particles. However, a broader range of materials and sample configurations must be examined before these promising results can be translated into a reliable and predictive in vitro biomaterials testing protocol. Modified glass slides were robotically printed with eight identical arrays consisting of capture antibodies against four mouse cytokines [IL-6, TNF-α, MIP-2, TGF-β1] and two positive and two negative detection controls. RAW 264.7 mouse monocytes seeded into six-well plates at 105 cells/well were treated with either sterilized Ti particles (test biomaterial), or lipopolysaccharide (LPS; positive control), or untreated (negative control). Aliquots (80 μl) of culture medium collected at 1, 6, 24, 48, and 72 h were applied to the protein arrays for simultaneous sandwich fluoroimmunoassay, followed by imaging the fluorescent intensities on a conventional microarray scanner. LPS induced the release of all four cytokines between 1 and 6 h treatment periods, whereas Ti induction of cytokines showed a gradual and subtle increase in cytokine expression for >24 h. Among the four cytokines assayed, TNF-α and MIP-2 were most prominently expressed, while IL-6 was slightly elevated and TGF-β1 was undetected above background.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 26, Issue 10, April 2005, Pages 1081-1085
نویسندگان
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