کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10230515 830 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo cytokine secretion and NF-κB activation around titanium and copper implants
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
In vivo cytokine secretion and NF-κB activation around titanium and copper implants
چکیده انگلیسی
The early biological response at titanium (Ti), copper (Cu)-coated Ti and sham sites was evaluated in an in vivo rat model. Material surface chemical and topographical properties were characterized using Auger electron spectroscopy, energy dispersive X-ray spectroscopy and interferometry, respectively. The number of leukocytes, cell types and cell viability (release of lactate dehydrogenase) were determined in the implant-interface exudate. The contents of activated nuclear transcription factor NF-κB, interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined by enzyme linked immunosorbent assay. An increase in the number of leukocytes, in particular, polymorphonuclear leukocytes, was observed between 12 and 48 h around Cu. A marked decrease of exudate cell viability was found around Cu after 48 h. The total amounts of activated NF-κB after 12 h was highest in Ti exudates whereas after 48 h the highest amount of NF-κB was detected around Cu. The levels of cytokine IL-6 were consistently high around Cu at both time periods. No differences in IL-10 contents were detected, irrespective of material/sham and time. The results show that materials with different toxicity grades (titanium with low and copper with high toxicity) exhibit early differences in the activation of NF-κB, extracellular expression and secretion of mediators, causing major differences in inflammatory cell accumulation and death in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 26, Issue 5, February 2005, Pages 519-527
نویسندگان
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