کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10255445 | 161733 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Forensic DNA typing strategy of degraded DNA on discarded cigarette ends using the AmpFâSTR® Identifiler®, Identifiler® Plus and MiniFiler⢠PCR Amplification Kits
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Forensic DNA typing strategy of degraded DNA on discarded cigarette ends using the AmpFâSTR® Identifiler®, Identifiler® Plus and MiniFiler⢠PCR Amplification Kits Forensic DNA typing strategy of degraded DNA on discarded cigarette ends using the AmpFâSTR® Identifiler®, Identifiler® Plus and MiniFiler⢠PCR Amplification Kits](/preview/png/10255445.png)
چکیده انگلیسی
DNA left on a forensic sample is often prone to degradation, especially if left to the elements. To maximize the chance of retrieving the most information from such compromised DNA, an appropriate profiling scheme using the available technologies needs to be devised. In this study, a total of 62 cigarette ends collected under different conditions of environmental exposure were employed to test the effectiveness of three DNA amplification kits, namely the Applied Biosystems⢠AmpFâSTR® Identifiler®, Identifiler® Plus and MiniFiler⢠PCR Amplification Kits, in the profiling of such compromised DNA. We demonstrated that Identifiler® Plus could substitute Identifiler® to improve the effectiveness of profiling for those inhibited cigarette samples. MiniFilerâ¢, on the other hand, could supplement Identifiler®/Identifiler® Plus profiles and provide additional genetic information to enhance the evidential value of the samples, especially for those that have suffered from DNA degradation to a greater extent. The findings in this work allowed us to propose a DNA profiling strategy as follow: 1) samples yielding complete Identifiler®/Identifiler® Plus profiles require no further testing with MiniFilerâ¢; 2) samples yielding partial single-source profiles to be tested with MiniFiler⢠to add genetic information; 3) samples yielding no results are unlikely to yield any results with MiniFilerâ¢.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science & Justice - Volume 54, Issue 4, July 2014, Pages 311-315
Journal: Science & Justice - Volume 54, Issue 4, July 2014, Pages 311-315
نویسندگان
Stephen C.Y. Ip, Sze-wah Lin, Christina Li, Kam-ming Lai,