کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10302453 | 542940 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glucocorticoid receptor gene expression and promoter CpG modifications throughout the human brain
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کلمات کلیدی
AMGVMHvlmethylation statusSFGSOGSPGITGPHGIFGMFGMTGAmygdala - آمیگدال، بادامهsubstantia nigra - توده سیاهdentate gyrus - شکنج دندانه دارPituitary gland - غده هیپوفیزPineal gland - غده پینه آلInferior temporal gyrus - قارچ قاعدگی پایینSuperior frontal gyrus - قارچ پیشانی فوقالعادهinferior frontal gyrus - قارچ پیشانی پایین ترPUT - قرار دادنlocus coeruleus - لوکوس سیرولئوسwhite matter - ماده سفیدPVN - مالیات بر ارزش افزودهCerebellum - مخچهHuman brain - مغز انسانNucleus accumbens - هسته accumbenscaudate nucleus - هسته دم دارparaventricular nucleus - هسته پروژسترویکPutamen - پوسته، پوتامنmiddle temporal gyrus - گریش زمان متوسطGlucocorticoid - گلوکوکورتیکوئیدهاPit - گودالcingulate gyrus - گودرزیmiddle frontal gyrus - گوریور پیشانی متوسطGlobus pallidus - گوی رنگ پریده، گلوبوس پالیدوسMineralocorticoid receptor - گیرنده مینرالوکورتیکوئید Mineralocorticoid receptors - گیرنده های Mineralocorticoidglucocorticoid receptors - گیرنده های گلوکوکورتیکوئیدglucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
روانپزشکی بیولوژیکی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glucocorticoids and the glucocorticoid (GR) and mineralocorticoid (MR) receptors have been implicated in many processes, particularly in negative feedback regulation of the hypothalamic-pituitary-adrenal axis. Epigenetically programmed GR alternative promoter usage underlies transcriptional control of GR levels, generation of GR 3Ⲡsplice variants, and the overall GC response in the brain. No detailed analysis of GR first exons or GR transcript variants throughout the human brain has been reported. Therefore we investigated post mortem tissues from 28 brain regions of 5 individuals. GR first exons were expressed throughout the healthy human brain with no region-specific usage patterns. First exon levels were highly inter-correlated suggesting that they are co-regulated. GR 3Ⲡsplice variants (GRα and GR-P) were equally distributed in all regions, and GRβ expression was always low. GR/MR ratios showed significant differences between the 28 tissues with the highest ratio in the pituitary gland. Modification levels of individual CpG dinucleotides, including 5-mC and 5-hmC, in promoters 1D, 1E, 1F, and 1H were low, and diffusely clustered; despite significant heterogeneity between the donors. In agreement with this clustering, sum modification levels rather than individual CpG modifications correlated with GR expression. Two-way ANOVA showed that this sum modification was both promoter and brain region specific, but that there was however no promoter*tissue interaction. The heterogeneity between donors may however hide such an interaction. In both promoters 1F and 1H modification levels correlated with GRα expression suggesting that 5-mC and 5-hmC play an important role in fine tuning GR expression levels throughout the brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Psychiatric Research - Volume 47, Issue 11, November 2013, Pages 1597-1607
Journal: Journal of Psychiatric Research - Volume 47, Issue 11, November 2013, Pages 1597-1607
نویسندگان
Lei Cao-Lei, Songkiet Suwansirikul, Prapan Jutavijittum, Sophie B. Mériaux, Jonathan D. Turner, Claude P. Muller,