A constrained independent component analysis technique for artery-vein separation of two-photon laser scanning microscopy images of the cerebral microvasculature

► Data driven and model based techniques are combined in the constrained ICA (CICA). ► Gamma variate model-following constraint is added to ICA in temporal domain. ► Non-negativity constraint is added to the ICA spatial domain. ► CICA performed better than data-driven technique (ICA) in simulation and experiment. ► CICA outperformed model based technique (gamma Model) in simulation and experiment.