Assessment of acrosome state in boar spermatozoa heads using n-contours descriptor and RLVQ

This paper proposes a method for assessing the acrosome state of boar spermatozoa heads using digital image processing. We use gray level images in which spermatozoa have been labeled as acrosome-intact or acrosome damaged using the information of a coupled fluorescent image. The heads are segmented obtaining the outer head contour. A set of “n” inner contours separated by a logarithmic distance function is calculated later. For each point of the, in this case, seven contours a number of local texture features are computed. We have compared the classification performance of Relevance Learning Vector Quantization, class conditional means and KNN, employing cross-validation for the evaluation. Gradient magnitude data offer the best result with an overall test error of only 1%. This result outperforms previously applied methods and suggests this approach as an interesting automatized approach to this veterinarian problem.