Influence of dipalmitoylphosphatidylcholine (or dioleoylphosphatidylcholine) and phospholipase A2 enzyme on the properties of emulsions

► The behavior of a zwitterionic lipid layer on oil droplets is strictly dependent on the lipid molecular structure. ► Na+ and Ca2+ ions bound to the headgroups of phospholipid can affect the lipid order ► Ions act to increase attractions between the neighboring lipids and decrease the emulsion effective diameter. ► Phospholipid hydrolysis by PLA2 is enhanced by added ethanol. ► The effect of ethanol on n-tetradecane/DOPC emulsions is comparable with that when DPPC is used as stabilizer.