Effectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposome

► We designed a new synthetic peptide (NLS) to act as a nuclear localization signal. ► The peptide was included in DNAhsp65/cationic liposome as a gene vaccine. ► The peptide/DNAhsp65 complex was inserted into the liposome structure. ► Peptide inclusion presented in vivo therapeutic effects against tuberculosis. ► The pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment.