Multilayer film assembled from charged derivatives of chitosan: Physical characteristics and biological responses

► LBL assembly of charged derivative of chitosan was constructed. ► All multilayer films are relatively rigid and have high water content associated. ► Cytocompatibility of multilayer films depends upon the outermost layer. ► The HTACC/PAA showed moderate antibacterial activity against Escherichia coli and Staphylococcus aureus. ► Strong anticoagulant activity was observed on the multilayer films terminating with SFC.