Aggregation behavior of aqueous dioctadecyldimethylammonium bromide/monoolein mixtures: A multitechnique investigation on the influence of composition and temperature

► Monoolein content strongly influences the structure of DODAB/MO mixtures. ► DODAB/MO mixtures present a two-region phase diagram, above and below χDODAB = 0.5. ► Temperature increase mimics the effect of higher MO content on aggregate morphology. ► Both MO content and temperature can be tuned up to optimize nucleic acid delivery.