Size-controlled and monodisperse enzyme-encapsulated chitosan microspheres developed by the SPG membrane emulsification technique

► Prepared lysozyme-encapsulated chitosan microspheres by SPG membrane emulsification. ► Achieved monodispersity and size-controllability of the microspheres. ► Could predict the average diameters of the microspheres with no fitting parameters. ► Controlled the amount of encapsulated lysozyme. ► Elucidated the maintenance of the activities of the encapsulated lysozymes.